Composite

Part:BBa_K1124107:Design

Designed by: Takahiro Yamada   Group: iGEM13_UT-Tokyo   (2013-09-15)

pLac-hpaBC-plambda-sRNA(anti-tyrR)-plambda-sRNA (anti-csrA) (L-DOPA synthesis device)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 160
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1940


Design Notes

In this composite part, we chose the phage lambda pR promoter(Part:BBa_R0051) as the promoter of sRNA because the transcriptional start site of pR promoter was well determined, and its operator site is embedded within the pR promoter. These characteristics strongly suggest that no dummy nucleotides will be attached to the sRNA.



References

Yoo, S. M., Na, D., & Lee, S. Y. (2013). Design and use of synthetic regulatory small RNAs to control gene expression in Escherichia coli. Nature protocols, 8(9), 1694-1707.